Transdermal Application of Mutant HSP70i
Session Number
Project ID: MEDH 22
Advisor(s)
Dr. Le Poole; Northwestern University
Discipline
Medical and Health Sciences
Start Date
19-4-2023 8:50 AM
End Date
19-4-2023 9:05 AM
Abstract
Heat shock proteins (HSPs) are a group of proteins involved in protecting cells from damage. HSP70 is a type of HSP that is found in high levels in various types of cells, playing an important role in the regulation of protein folding, stabilization, and the prevention of protein aggregation. Recent research has suggested that the expression of is reduced in vitiligo patients, which may contribute to the destruction of melanocytes, the cells that produce pigment in the skin. In TCR transgenic mouse models of vitiligo, it has been shown that the introduction of HSP70iQ435A can prevent and treat the disease. This has led to an interest in exploring the potential use of HSP70iQ435A as a potential treatment for vitiligo in humans. This project introduces HSP70iQ435A in both DNA and protein formats using electroporation, a titanium micro roller, and a gene gun, for the introduction of DNA as a range of options for drug delivery. The proposed method of detecting the tagged HSP70iQ435A is through immunohistology using various HSP70 primary antibodies and secondary antibodies. This provides insight into the distribution of the drug in the skin and its potential effectiveness as a treatment for vitiligo and melanoma.
Transdermal Application of Mutant HSP70i
Heat shock proteins (HSPs) are a group of proteins involved in protecting cells from damage. HSP70 is a type of HSP that is found in high levels in various types of cells, playing an important role in the regulation of protein folding, stabilization, and the prevention of protein aggregation. Recent research has suggested that the expression of is reduced in vitiligo patients, which may contribute to the destruction of melanocytes, the cells that produce pigment in the skin. In TCR transgenic mouse models of vitiligo, it has been shown that the introduction of HSP70iQ435A can prevent and treat the disease. This has led to an interest in exploring the potential use of HSP70iQ435A as a potential treatment for vitiligo in humans. This project introduces HSP70iQ435A in both DNA and protein formats using electroporation, a titanium micro roller, and a gene gun, for the introduction of DNA as a range of options for drug delivery. The proposed method of detecting the tagged HSP70iQ435A is through immunohistology using various HSP70 primary antibodies and secondary antibodies. This provides insight into the distribution of the drug in the skin and its potential effectiveness as a treatment for vitiligo and melanoma.