Role of Matrix Metalloproteinase Inhibitors on CMT-93 Wound Healing
Session Number
Project ID: MEDH 23
Advisor(s)
Dr. Ronen Sumagin; Northwestern University, Feinberg School of Medicine
Discipline
Medical and Health Sciences
Start Date
19-4-2023 10:20 AM
End Date
19-4-2023 10:35 AM
Abstract
Matrix metalloproteinase plays an important role in wound tissue regeneration, altering the wound matrix and mediating important responses to growth including angiogenesis and vasodilation. In specific, MMP2, MMP9, and MMP14 play major roles in wound healing through the regulation of angiogenesis and activation of proangiogenic cytokines as well as regulating the influx of immune cells. To test and see how MMPs affect wound healing, CMT-93s were grown and scratched using a P200 micropipette tip to administer a serum of DMEM complete media, MMP inhibitors (ranging from MMP2, MMP9, and MMP14), and placed in treated 24-well plates. Intervals of 8 to 14 hours were counted and after, images were taken to see how relative wound density and cell migration were affected by different inhibitors. Analysis from Fiji Software revealed that MMP2i (MMP2 inhibitor) was proved to have the greatest cell migration across the wound space as well as high values in relative wound density (the percentage of tissue regenerated compared to the original wound area) followed by MMP9i and MMP14i that had similar data; vehicle subject showed significant data as well. This data demonstrates the effect MMP inhibitors could potentially have on future wound growth in cancerous tissue.
Role of Matrix Metalloproteinase Inhibitors on CMT-93 Wound Healing
Matrix metalloproteinase plays an important role in wound tissue regeneration, altering the wound matrix and mediating important responses to growth including angiogenesis and vasodilation. In specific, MMP2, MMP9, and MMP14 play major roles in wound healing through the regulation of angiogenesis and activation of proangiogenic cytokines as well as regulating the influx of immune cells. To test and see how MMPs affect wound healing, CMT-93s were grown and scratched using a P200 micropipette tip to administer a serum of DMEM complete media, MMP inhibitors (ranging from MMP2, MMP9, and MMP14), and placed in treated 24-well plates. Intervals of 8 to 14 hours were counted and after, images were taken to see how relative wound density and cell migration were affected by different inhibitors. Analysis from Fiji Software revealed that MMP2i (MMP2 inhibitor) was proved to have the greatest cell migration across the wound space as well as high values in relative wound density (the percentage of tissue regenerated compared to the original wound area) followed by MMP9i and MMP14i that had similar data; vehicle subject showed significant data as well. This data demonstrates the effect MMP inhibitors could potentially have on future wound growth in cancerous tissue.