Testing the overexpression of a PNCTR through PNCs and the effect of the knockdown expression of proteins on PNC prevalence
Session Number
Project ID: BIO 06
Advisor(s)
Dr. Sui Huang; Northwestern University, Feinberg School of Medicine
Discipline
Biology
Start Date
19-4-2023 10:20 AM
End Date
19-4-2023 10:35 AM
Abstract
We are conducting experiments to investigate the effects of overexpressing a long-non coding RNA, known as PNCTR, in cells that typically exhibit low expression levels of PNCs, a metastatic cancer marker. Our objective is to determine whether augmenting the expression of PNCTR in these cells can increase the number of PNCs present. Our research is motivated by a prior study in which the authors observed a decrease in the quantity of PNCs present upon the elimination of PNCTR in cancer cells. Therefore, we seek to investigate whether the opposite scenario, where the introduction of additional PNCTR into cells, would result in an increase in PNCs. Additionally, we are concurrently screening a set of proteins to observe any changes in PNC prevalence when we reduce the levels of these protein expressions in cells. We aim to assess whether reducing the expression of these proteins could decrease the quantity of PNCs present in cells that typically express high levels of PNCs. This investigation will provide insight into the proteins contributing to the expression of PNCs and metastasis.
Testing the overexpression of a PNCTR through PNCs and the effect of the knockdown expression of proteins on PNC prevalence
We are conducting experiments to investigate the effects of overexpressing a long-non coding RNA, known as PNCTR, in cells that typically exhibit low expression levels of PNCs, a metastatic cancer marker. Our objective is to determine whether augmenting the expression of PNCTR in these cells can increase the number of PNCs present. Our research is motivated by a prior study in which the authors observed a decrease in the quantity of PNCs present upon the elimination of PNCTR in cancer cells. Therefore, we seek to investigate whether the opposite scenario, where the introduction of additional PNCTR into cells, would result in an increase in PNCs. Additionally, we are concurrently screening a set of proteins to observe any changes in PNC prevalence when we reduce the levels of these protein expressions in cells. We aim to assess whether reducing the expression of these proteins could decrease the quantity of PNCs present in cells that typically express high levels of PNCs. This investigation will provide insight into the proteins contributing to the expression of PNCs and metastasis.