Investigating the Role of TET2 Enzymes in the Proliferation, Differentiation, and Survival of Hematopoietic Stem Cells
Session Number
Project ID: BIO 08
Advisor(s)
Dr. Vipul Shukla; Northwestern Univeristy, Feinberg School of Medicine
Dr. Ajay Abraham; Northwestern Univeristy, Feinberg School of Medicine
Discipline
Biology
Start Date
19-4-2023 10:50 AM
End Date
19-4-2023 11:05 AM
Abstract
Ten-Eleven Translocation (TET) enzymes play a critical role in DNA demethylation, which is essential for mammalian development and differentiation. Recent research suggests that the absence of TET enzymes is associated with an increased risk of malignancies. Among the TET enzymes, TET2 is the most commonly mutated and the focus of our investigation. Using gene editing techniques based on clustered regularly interspaced short palindromic repeat (CRISPR), we aim to study the impact of TET enzyme removal from hematopoietic stem cells in mice. Our study builds on the findings of Dr. Vipul Shukla, whose research has shown that TET deficiency disrupts mature B cell homeostasis, leading to oncogenesis characterized by the accumulation of G-quadruplex and R-loop structures. Through our work, we hope to gain a deeper understanding of the role TET enzymes play in the proliferation, differentiation, and survival of hematopoietic stem cells in mice and identify potential therapeutic strategies.
Investigating the Role of TET2 Enzymes in the Proliferation, Differentiation, and Survival of Hematopoietic Stem Cells
Ten-Eleven Translocation (TET) enzymes play a critical role in DNA demethylation, which is essential for mammalian development and differentiation. Recent research suggests that the absence of TET enzymes is associated with an increased risk of malignancies. Among the TET enzymes, TET2 is the most commonly mutated and the focus of our investigation. Using gene editing techniques based on clustered regularly interspaced short palindromic repeat (CRISPR), we aim to study the impact of TET enzyme removal from hematopoietic stem cells in mice. Our study builds on the findings of Dr. Vipul Shukla, whose research has shown that TET deficiency disrupts mature B cell homeostasis, leading to oncogenesis characterized by the accumulation of G-quadruplex and R-loop structures. Through our work, we hope to gain a deeper understanding of the role TET enzymes play in the proliferation, differentiation, and survival of hematopoietic stem cells in mice and identify potential therapeutic strategies.